Abstract: Microbial pesticides have evolved as an alternate technique to chemical insecticides due to their high target specificity and ecological safety. Investigations were carried out to explore the efficacy of 28 actinobacterial extracts against a day old second instar larvae of Plutella xylostella, Spodoptera litura and Spodoptera frugiperda. Among the actinobacterial extracts, DBT-64 recorded 78.85, 76.50 and 76.00 per cent mortality of P. xylostella, S. litura and S. frugiperda respectively followed by DBT-80 with 80.75, 75.25 and 77.50 per cent mortality. Further, DBT-59 has recorded 79.50, 78.25 and 75.25 per cent larval mortality of P. xylostella, S. litura and S. frugiperda respectively at 72 HAT under in-vitro conditions. Among the actinobacterial extracts, three potent extracts (DBT-64, DBT-80 and DBT-59) were taken forward to greenhouse conditions to test verify their efficacy against P. xylostella, S. litura and S. frugiperda. DBT-80 has caused highest larval mortality in P. xylostella (84%), S. litura (82.75%) and S. frugiperda (80.50%) followed by DBT-64 with 81.00, 80.00 and 79.50 per cent larval mortality in P. xylostella, S. litura and S. frugiperda respectively at 96 HAT under green house condition. Among the isolates, DBT-80 showed higher insecticidal activity against the test insects followed by DBT-64 and DBT-59. These potential actinobacterial extracts can be used in the Integrated Pest Management programmes of Plutella xylostella, Spodoptera litura and Spodoptera frugiperda which are found very effective under laboratory and greenhouse condition.