Abstract : Rice (Oryza sativa L.) is a cereal grain, it is the most widely consumed staple food for a large part of the world’s human population, especially in Asia and Africa. Rice production is often subjected to several biotic and abiotic stresses rice sheath blight is one of the most destructive diseases causing great damage to rice yield and quality worldwide.Twenty rhizospheric soil samples of rice obtained during survey from different regions of Northern Karnataka were used for the isolation of Trichoderma spp. and fluorescent Pseudomonads. The antagonistic potential was studied by using dual culture technique. Among twenty Trichoderma spp., the highest inhibition (> 50%) was recorded in nine isolates which ranged from 54.20 to 65.10 per cent. The results obtained were highly significant between the different isolates and also over control. Among the twenty fluorescent Pseudomonads, the per cent inhibition was higher (> 50%) in twelve isolates which varied from 51.92 to 81.79 per cent. The results obtained were highly significant between the different isolates and also over control.