A field experiment was carried out to study the effect of sulphur, zinc and iron nutrition on growth, yield, nutrient uptake and quality of safflower on Vertisol at the Main Agricultural Research Station, (MARS) University of Agricultural Sciences, (UAS) Dharwad, during rabi season 2002-03. The results indicated that application of 30 kg S per ha improved the growth parameters like plant height, number of leaves, number of branches and dry matter, yield components viz., number of capsules, seed weight per head, 1000-seed weight and nutrient uptake of N, P, K, S, Zn and Fe as compared to other treatments. Combined application of sulphur along with micronutrients (Zn and Fe) had significant influenced on the growth, yield and nutrient uptake by safflower. The treatment receiving 30 kg S per ha + Fe + Zn foliar recorded the highest growth, yield and nutrient uptake as compared to 30 kg S per ha, 20 kg S per ha + Fe+ Zn foliar, 10 kg S per ha + Fe + Zn foliar spray and control.