Papaya is one of the important fruit crop of India. Cultural studies of Colletotrichum gloeosporioides causal agent of anthracnose of papaya on different solid media showed that the V-8 juice agar and Richard’s agar were good for growth and sporulation. The fungus in liquid medium there was a increase in growth upto ten days ofincubation and there was decrease in growth for eleventh day onwords. Temperature requirement of the fungus was found the 300C where good growth was observed. The fungus exposed to alternate cycles of light and darkness produced maximum growth and sporulation when compared to continuous light and continuous darkness.