The differences in their growth of four isolates of Macrophomina phaseolina (Tassi) Goid., upon incubation were studied. The maximum mean mycelial weight of 231.0 mg was recorded on 18th day of incubation and the isolate ICRISAT recorded highest mycelial weight (190.9 mg) and remained significantly superior to other isolates. Potato Dextrose Agar (PDA) and Czapek’s agar media were found best for the growth of all the isolates (88.4 mm each). The sclerotia production was maximum on PDA (123.3/microscopic field of 10x). The ICRISAT isolate recorded maximum sclerotia production (71.2), followed by Bidar isolate (68.68).