Sesame genotypes (64) were evaluated for nine yield and yield attributing characters to study the genetic diversity existing among them by using Mahalanobis D2 statistics. Analysis of variance revealed significant difference among genotypes for all the nine character studied. Based on the D2 values  the genotypes were grouped into nine different clusters. The genotypes grouped into various clusters revealed that there was no relationship between geographical distribution and genetic diversity. Maximum inter cluster distance was observed between cluster VIII and cluster IV (25.24) while, lowest divergence was noticed between cluster I and VII (10.23). Among the nine characters studied, 1000 seed weight contributed highest towards genetic divergence (38.26 %) followed by number of capsules per plant (28.50 % ) and seed yield per plant ( 19.42 %). Cluster IV exhibited highest means for plant height, number of branches per plant, number of seeds per capsule and capsule length. Cluster VIII exhibited lowest means for plant height, number of branches per plant and capsule length. The genotypes from cluster IV and VIII which have high and low cluster means for the majority of the characters are suggested as parents for hybridization programme to achieve novel recombinants.