A total of 10 yeast strains were isolated from their ability to grow on 2% glucose medium. Efficiency was tested on the basis of growth, ethanol tolerance, fermentation, assimilation of carbon sources, sugar utilization and alcohol production. The standard strain of Saccharomyces ellipsoideus No. 101 recorded highest growth with OD values of 0.396 to 1.643 at 12 to 48 hours. The assimilation of lactose was recorded by all strains. Addition of 5% ethanol at initial stage, the strain FWY-4 recorded highest growth with OD values of 0.418 and 0.928 at 24 and 48 hour, respectively whereas initial addition of 7.5% ethanol recorded highest OD by S. ellipsoideus No. 101 (0.229 and 0.463 at 24 and 48 hours, respectively). Slow fermentation was recorded by strains FWY-2 and FWY- 10. Alcohol production was highest in case of reference strain S. ellipsoideus No. 101 (8.36%) followed by FWY-4 (8.25%) and FWY-6 (7.80%). This study showed that the isolated yeast strains like FWY-4 and FWY-6 performed better with all parameters studied but however found superior only next to standard strain No. 101 of S. ellipsoideus.