Eight different cotton (G. hirsutum) hybrids and varieties commonly grown in Karnataka were collected and their leaf, pod, stem and root were separated. Average values of analysis of 8 varieties of different parts and the whole cotton plant were subjected for chemical analysis. The chemical composition and antinutritional factors among the different varieties of cotton plant and parts did not vary significantly. However, the littfe variations observed could be due to plant size and stage of maturity at harvest. Significantly (P<O.05) higher CP, EE, NFE, Cell contents, total ash and Ca and lower CF, NDF, ADF and cellulose were observed in cotton leaf than pod, stem and root. Significantly (P<O.05) higher CP and EE and lower CF in seed than whole cotton plant was observed; whereas the difference among the pod, stem, root and whole cotton plant was not significant. Silica was more in pod than leaf, stem, root & seed and Lignin was more in stem and seed than leaf, pod and root. Tannin was more in leaf and pod than stem, root and seed. Gossypol was more in local varieties followed by improved varieties and hybrids. Seed contained more gossypol compared to leaf, pod, stem and root. It was observed that the chemical composition of cotton plant was comparable to cereal straws and the antinutritional factors were well within the safety limits. The cotton plant, after harvesting cotton can very well be incorporated in the ruminant ration. However, the level and form of inclusion have to be studied.