Fifty two chilli genotypes were screened against fruit rot of chilli caused by colletotrichum capsici (Sydow) Butler and Bisby under natural conditions at Agricultural Research Station, Hanumanmatti. LCA-301, LCA-324, K-1 and Byadgi Kaddi were found resistant and KDSC-210-10 and S-32 were found highly susceptible. Resistant genotypes contained higher capsaicin ascorbic acid and lower total sugars than susceptible varieties.