Twelve sorghum (Sorghum bicolor L. Moench) cultivars including nine varieties, one hybrid and its parental lines were discriminated by seed and seedling morphology and chemical response tests. All twelve cultivars showed variation in the seed descriptors viz., seed colour, seed size, seed shape, size of mark of germ, texture of endosperm, colour of viterous albumin and seed lusture and seedling descriptors viz., anthocyanin pigmentation on coleoptile, dorsal side of first leaf, sheath of first leaf and on leaf blade (at five leaf stage). The results of the chemical tests obviously revealed that the hybrid and its parents responded differently to various chemical tests. The hybrid COH4 showed negative to both standard phenol and modified phenol test (CUSO4, 0.4%) whereas, it reacted as light yellow to KOH test as well showed high response to GA3. The female parent MS296A showed negative reaction to standard phenol test. However it responded as light brown colour reaction to modified phenol (CUSO4) test. The male parent TNS 30 showed light brown in standard phenol test. while it responded as negative to modified phenol (Na2CO3, 0.6%) test.