An Investigation pertaining to Evaluation of vitamin A Prophylaxis Programme was carriedout in rural areas of Dharwad taluka. An attempt was made to assess the knowledge of functionaries who were operating the prophylaxis programme and to evaluate on-going prophylaxis programme in selected three PHCs. The results revealed that the medical officer had better knowledge scores (83.30%) followed by DHO (77.71 %) and ANSs (63.30%) with respect to vitamin A prophylaxis programme. All functionaries had well conceived knowledge about the objective of the programme, dosage, frequency, method and place of distribution. On the contrary, none of them had knowledge about the exact year of implementation of the vitamin A prophylaxis programme and cost of vitamin A doses.