Effects of inoculation with arbuscular mycorrhizal fungi (AMF) Acaulospora laevis, Glomus fasciculatum and Sclerocystis dussii, to induce positive effects on secondary hardening and growth of plantlets of Gerbera (Gerbera jamesonii) cv. Pink Elegance and Danaellen, propagated in vitro, were studied under shade net. Among the mycorrhizal fungi studied, Sclerocystis dussii was the most efficient species in influencing the plant growth characters and cv. Danaellen exhibited better growth parameters compared to Pink Elegance. Sclerocystis dussii inoculated plantlets showed 17 percent, 48.47 percent and 33.8 per cent increase in shoot length, root length and shoot P content respectively when compared to uninoculated plantlets. This study convincingly demonstrates that the mycorrhizal association helps in successful establishment and growth of tissue culture raised plantlets of gerbera during secondary hardening by alleviating the transplanting shock.