The palpebral conjunctiva of the water buffalo was more extensive than the bulbar conjunctiva. There were no goblet cells or any lymphocytic aggregation in the conjunctiva, which was quite contrary to other species. The corneal transparency was mainly maintained by the hollow spaces seen in the stroma as the precorneal tear film was not evident. The hollow spaces evident in the corneal epithelium may be nerve ending or fixation artefact. Absence of pectinate ligament and canal of Schlemm, leads to the speculation that scleral plexus is the potential route for the out flow of aqueous humor in the buffalo eye. The centroperipheral thickening of the cornea was the unique species character of buffalo eye.