Arbuscular mycorrhizal (AM) fungal associations in majority of terrestrial plants are universal. AM fungal association on amaranthaceae considered to be disputed. The present studies enlist the prevalence of AM fungal colonization in amaranthaceae. Field studies were undertaken to screen AM fungal association and isolate interesting AM fungal spores. Twenty three plants have screened for AMF which were grown in agricultural fields. Altogether thirty five indigenous AM fungal spores are recovered from this study. There was variation in the mycorrhizal colonization and spore number. Therefore, the present study revealed that the genus Glomus was more predominant than others and Scutellospora was least amongst the recovered AMF spores. The presence fungal components in the roots and the spore number in the soil has brought possible mycorrhizal association with varied per cent of colonization in members of Amaranthaceae