An experiment was conducted to assess the genetic divergence among nine Alphonso clone of mango, collected from different pickets of both Maharashtra and Karnataka states in India, using Mahalanobis D2 statistic over physico-chemical parameters of fruits. Analysis of variance revealed that high significant differences among clones existed for all characters except stone thickness. The clones were grouped into two clusters. Cluster I, which consists of the clones DPL-I, RTN-I, DEV-1, VEN-1, DWR-I, DWD-II, DWD-III and BGM-II had the highest length of fruit (14.37 cm), stone thickness (2.24 cm),percentage of pulp (71.58), pulp to stone ratio (4.75:1), pulp to peel Ratio (5.21:1) and shelf life (19.85 days). Cluster-II, comprising of the clones BGM-1 recorded significantly highest total fruit weight 260.8 g), diameter of fruit (70.22 cm), weight of peel (40.79 g), stone weight (41.88 g) and weight of pulp (178.46 g). Regarding quality parameters, the highest values were observed for total soluble solids as (16.69 0 B), ascorbic acid (54.92 mg/100 g) and total sugar (13.42 mg/100 g) in cluster-I. Similarly in cluster-II. The highest values were recorded in titrable acidity (0.56%) and reducing sugar (3.81 mg/100 g). No relationship between geographical origin and genetic diversity was established. Selecting clones from divergent clusters and utilizing them in hybridization programme is likely to produce desirable segregant in subsequent generations and may lead to improvement in Alphonso mango for yield and quality traits.