The studies on microrhizome production in turmeric was carried out at the tissue culture laboratory, Departmentof Horticulture, University of Agricultural Sciences, Dharwad during 2006-08. This study on development of protocol for microrhizome production included influence of growth regulators, medium strength and photoperiod. Best response was obtained in medium containing BAP 1mg/l with NAA 0.2 mg/l (31.33 days) followed by BAP 1 mg/l alone (43.33 days) for early induction of microrhizomes. More number of microrhizomes and bigger microrhizomes were produced in MS medium having lower concentration of BAP. The study also revealed that full strength MS medium recorded higher number of microrhizomes, weight of microrhizomes and nodes per microrhizome which was followed by half strength medium. Further, intraction between photoperiod condition and media types showed that dark condition with liquid and semisolid medium produced highest number of microrhizomes per shoot (7.00), followed by four hour light condition with liquid and semisolid medium.