An experiment was conducted to study the effect of salt stress on growth parameters in three cotton genotypes viz. Bikeneri Nerma, AC-738 and Jayadhar under in vitro condition. The salinity stress was induced by incorporating different concentrations of salts to generate EC (electric conductivity) levels 2, 4, 6, 8 and 12ds / m referred to as T1 to T5  respectively. Genotypes showed differential response to different EC levels. Lesser per cent reduction of seedling fresh weight, shoot length, root length, root dry weight was observed in Bikeneri Nerma than Jayadhar and AC-738. The root growth was completely inhibited in AC-738 beyond EC level of 8. However, in Bikeneri Nerma and Jayadhar root growth was observed in EC levels of 8 and 12. It indicates that Bikeneri Nerma and Jayadhar may be salt tolerant than AC-738.