Plant growth regulators GA3, paclobutrazol and maleic hydrazide were tried at three different concentrations in tuberose (cv. Single) to evaluate and arrive at the best concentration that aids in realising higher flower production in tuberose. GA3 increased the plant height, number of leaves, number of shoots and leaf area, whereas maleic hydrazide and paclobutrazol reduced all these parameters compared to control.  Flowering was enhanced by GA3. Spraying GA3 at 150 ppm, paclobutrazol at 1500 ppm followed by maleic hydrazide 1000 ppm at 30 and 60 days after planting is ideal to realise higher flower spike yield, whereas spraying GA3 at 150 ppm has realized maximum loose flower yield.