Cross inoculation studies involving five crops (groundnut, maize, sorghum, safflower and soybean) and the respective isolates of Macrophomina phaseolina in addition to the oilpalm isolate showed that all the six isolates were pathogenic and cross inoculable to all the five host plants inoculated. The effect of different crops, different concentrations (4 and 88) and variation in different isolates and their interactions were found to be highly significant. Oilpalm isolate was found to be highly virulent whereas safflower and groundnut were found to be the most susceptible hosts. Soybean isolate was found to be the least virulent on sorghum, while sorghum isolate was found to be the least virulent on soybean host. Oilpalm isolate was identified as highly virulent, while sorghum, maize, groundnut, safflower and soybean were grouped under virulent isolates