The pathogen Colletotrichum gloeosporioides produced non specific toxic metabolites in culture filtrate. Drooping and wilting were the striking symptoms observed in most of the plant species tested in culture filtrate but the time taken for expression symptoms varied from plant to plant. Maximum amount of toxin was produced on 16th day of incubation. Culture filtrate inhibited the seed germination of coriander, tomato and radish drastically. The toxin proved to be thermostable and retained its toxioity even after autoclaving at 1.1 kg/cm2 pressure for 15 minutes, while, by diluting with water, its toxicity was reduced to a great extent The dilutions of the culture filtrate and the time taken for symptom expression were positively related.