Emmer wheat (Triticum dicoccum (Schrank.) Schulb.) is known for its high protein and dietary fiber contents but is non-free threshable and highly susceptible to spot blotch caused by Bipolaris sorokiniana (Sacc.) Shoemaker. We have crossed potential emmer wheat lines and subjected to mutagenesis to isolate free threshable and leaf blight resistant lines. Fifty-five free threshable mutant emmer wheat lines thus derived were evaluated for spot blotch resistance in the present study. Four free threshable mutant lines viz., NP-200 x DDK 1009-1, NP-200 x DDK-1009-9, NP-200 x MACS-2912-1 and DDK-1013x DDK-1001-3 were found highly resistant to spot blotch disease. Compared to susceptible genotypes, resistant genotypes recorded higher amount of total phenol, total protein, and reducing sugars but lesser number of stomata.