A total of 92 isolates of fluorescent pseudomonadswere collected from the rhizosphere soil of chilli, sunflower, redgram, groundnut, fieldbean, greengram, brinjal, tomato, burmuda grass, beans, sorghum, paddy and sesame from the farm of University of Agricultural Sciences, Raichur and surrounding villages, Dharwad and IIHR, Bangalore using specific medium. All the isolates showed Gram-negative reaction and were rod shaped, 66 isolates produced pigment in PAF medium and showed fluorescence under UV light. Two potential antagonistic isolates viz.,P. fluorescens (Pf4 and Pf6) were used for evaluating biocontrol efficacy. Among two isolates P. fluorescens ( Pf4 and Pf6), Pf4 showed maximum inhibition of mycelial growth of Macrophomina phaseolina (21.30%), Rhizoctoinia bataticola (24.07%), Rhizoctonia solani (32.96%), F. oxysporum .f. sp.udum (48.14%), C. gloeosporioides (41.67%), F.solani (24.07%), Sclerotiumrolfsii (40.74%), Cercospora capsicii (21.67%), Alternaria sesami (20.00%) and Xanthomonas axonopodispv. punicae (1.84 mm) through dual plate technique thus proved to be broad spectrum.