The present investigation was taken up at the tissue culture laboratory and greenhouse unit under the  Department of Horticulture during  1998-2000. Micropropagated plantlets of banana cultivars Dwarf Cavendish and Robusta were inoculated during the secondary hardening stage with arbuscular mycorrhizal fungi. All the AM fungi increased the growth of both the cultivars, but the positive influence of the fungi was more evident for Dwarf Cavendish plantlets inoculated with Glomus fasciculatum. The mycorrhizal fungi heavily colonized the root system of both the  cultivars. Plantlets of both the cultivars inoculated with. G.fasciculatum exhibited a higher plantlet height (60.7%  over control) and leaf area ( 2.2 times over control besides increasing the psuedostem girth (39.6%) over control and shoot biomass production. The symbiotic association also increased the shoot P concentration. Thus, mycorrhiza formation appears to be the key factor in improving the vigour and growth of micropropagated banana plantlets, which aids in the acclimatization process.