In vitro studies were conducted on the cultural and physiological features of the pathogen Xanthomonas oryzae pv. oryzae. The results indicated that out of ten solid media (five selective and five differential) tested for their efficacy to support the growth of X. oryzae pv. oryzae, Modified D-5 medium significantly supported the maximum growth of the pathogen as per the recovery of bacterial colonies (131.25 x 105 cfu/ml). Colonies of the2 pathogen were circular, flattened, slightly raised, convex with yellow to bright yellow colour on MD-5 and NA medium. The temperature of 30oC was found optimum for the maximum growth of the pathogen as witnessed by the highest recovery of bacterial colonies (154.33 x 105 cfu/ml) and pH of 7.0 was found optimum for the best growth of the pathogen (159.50 × 105 cfu/ml).