Journal of FARM SCIENCES, Vol 26, No 3 (2013)

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In vitro grafting in apple (Malus domestica. Borkh) cv. Lal Ambri

K.M. Bhatt, F.A. Banday, M.A. Mir, Z.A. Rather, G. Hussain

Abstract


This study was carried out to assess the potential use and applicability of micrografting technique for developing in vitro grafted plantlets. Microshoots of apple cv. Lal Ambri was used as scion and rooted microshoots of Malling-9 as rootstock. Different grafting methods and media formulations were investigated. Main effect of grafting methods and media formulation was found to be significant. Maximum grafting success of 29.01 % was achieved with vertical slit method, which decreased to 21.07 % with horizontal method of grafting. MS semi-solid medium with 3 % sucrose proved best with 29.47 % successful grafts, which decreased to 21.55 % when micrografts were cultured in MS liquid medium plus vermiculite with 6 % sucrose. Interaction effects were significant. Overall, best response of successful micrografts were obtained with a combination of vertical slit method of grafting and culturing micrografts  in  MS semi solid medium with 3 % sucrose, which showed highest graft success (35.76 %).

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