In this study an attempt has been made to optimize the dyeing conditions using natural colourant i.e. Mesta calyces extract. It was found that Acidic media (pH – 4), 45 minutes dye extraction time, 6 per cent dye concentration, 30 minutes dyeing time, 80° c dyeing temperature, pre mordanting time for Potash alum, Copper sulphate, Ferrous sulphate was 45 minutes and 15 minutes for Stannous chloride, 45 minutes simultaneous mordanting time for all the mordants and post mordanting time for Stannous chloride was 45 minutes and 30 minutes for Potash alum, Ferrous sulphate and 15 minutes for Copper sulphate were optimized at 400 wavelength(ë) for dyeing Mesta fibre with Mesta calyces extract.