A field experiment was conducted to study the effect of growth regulators on seed yield and quality in annual chrysanthemum (Chrysanthemum coronarium L.) during kharif 2008. Results revealed that spraying of Gibberellic acid @ 200 ppm induced early flowering followed by Gibberellic acid @ 100 ppm and similar trend was observed with tricontanol @ 1000 and 500 ppm. On the contrary, among the growth retardants, mepiquat chloride @ 1000 and 2000 ppm and cycocel @ 1000 and 2000 ppm delayed flowering as compared to the Gibberellic acid and tricontanol. Spraying of GA₃ @ 200 ppm significantly increased number of capitulum per plant, capitulum diameter, number of seeds per capitulum, dry weight of capitulum, 1000 seed weight and seed yield (per plant and per ha) as compared to control. Similarly, tricontanol @ 1000 and 500 ppm, mepiquat chloride@ 1000 and 2000 ppm and cycocel @ 1000 ppm and 2000 ppm also significantly improved the number of capitulum per plant, capitulum diameter, dry weight of capitulum, 1000 seed weight and seed yield (per plant and per ha) as compared to control. The seed quality parameters such as germination percentage, seedling length and vigour index and seedling dry weight were higher with lower electrical conductivity with GA₃ @ 200 ppm followed by GA₃ @ 100 ppm and similar observations were recorded with tricontanol @ 1000 ppm and 500 ppm. Among the growth retardants, mepiquat chloride @ 1000 ppm recorded significantly higher germination percentage, seedling length, vigour index and seedling dry weight with lower electrical conductivity followed by mepiquat chloride @ 2000 ppm and cycocel @ 1000 and 2000 ppm