To investigate the effect of continuous application of inorganic fertilizers and organic manures on yield, uptake and soil fertility, the Permanent Manurial Experiment (PME) has been initiated since 1909 in front of student’s hostel at Tamil Nadu Agricultural University, Coimbatore and being maintained till now. It is the oldest one having historical importance in India as it has crossed 100 years of continuous experimentation. Maize-sunflower cropping sequence is being followed under irrigated condition since 2008. Experiment consisted of 18 treatments as non replicated plots with plot size of 100 m2. The highest grain yield of 9906 kg ha-1 and straw yield of 12681 kg ha-1 was recorded with integrated application of 100% NPK along with farm yard manure @ 12.5 t ha-1 (INM practice) followed by STCR-IPNS. The INM practice proved its superiority by recording 10.4 per cent increase in grain yield of maize hybrid over 100% NPK. Total uptake of NPK was also found to be high in INM practice. Likewise, continuous application of 100% NPK along with FYM @ 12.5 t ha-1 resulted in the highest organic carbon (0.70%), available nitrogen (216 kg ha-1) and potassium (920 kg ha-1). However, available phosphorus was higher (32.14 kg ha-1) when poultry manure was applied continuously on nitrogen equivalent basis. The results also indicated that DTPA extractable Fe and Zn was found to be deficient in all the treatments whereas Cu and Mn seem to be sufficient in all the treatments.