A total of 320 cotton germplasm collections developed in various breeding schemes, commercially released varieties, exotic and indigenous germplasm collections were utilized to study the genetic variations for fiber quality traits by evaluating with five checks viz., Sahana, Surabhi, MCU-5, DS-28 and ARBH-813. The germplasm lines recorded high genetic and phenotypic coefficient of variation across five environments. In the present study, significant positive associations were observed between fiber length, tenacity and negative correlation with micronaire and uniformity ratio. Simple selection for long fiber and high fiber strength in the germplasm is possible. Present study also estimated genetic divergence based on D2 values, 320 germplasm lines including five checks were grouped into eighteen clusters based on fibre quality traits indicating high amount of diversity among the germplasm lines. For fiber quality traits, germplasm lines were grouped in to 18 clusters. Cluster XI was the largest with 41 germplasm lines. The 2.5% span length was the largest contributor with 36.76 per cent towards divergence. The germplasm lines grouped under I showed high tenacity, optimum micronaire and uniformity ratio. The lines under cluster II recorded extra long 2.5% span length (>32.5 mm).