Journal of FARM SCIENCES, Vol 29, No 3 (2016)

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Identification of polymorphic markers and introgression of Pi1 and Pi2 genes for blast resistance in rice

Shrinidhi S. Hegde, S.K. Prashanthi

Abstract


The most devastating diseases that constrain rice production is rice blast, caused by Magnaporthe oryzae which ranks first in threat to rice productivity. The use of resistant cultivars is the most effective and economical way to control rice blast disease. “Intan” is a medium slender indica variety, popular with farmers and consumers across India because of its yield and excellent popping quality. But, it is highly susceptible to blast disease.  Near isogenic lines (NILs) in BPT5204 background carrying blast resistant genes Pi1 and Pi2 were used as donor parent to introgress these genes into Intan variety using marker assisted backcrossing (MABC). Molecular markers linked to Pi1 and Pi2 genes were used to study parental polymorphism. Linked/Genic markers RM224 (0.0cM), RM1233 (0.0cM) for Pi1 gene and AP5930 (0.0cM), AP5659-5 (0.10cM) for Pi2 gene were polymorphic. F1 plants were generated independently for both the genes and hybridity was confirmed using linked polymorphic SSR markers. Further, these F1 plants were challenge inoculated with Magnaporthe oryzae isolates and F1s showed resistant reaction, confirming the hybridity.

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