Rice brown spot is one of the most important diseases of rice affecting the quality and quantity of  rice grains which causes severe yield reduction up to 53 per cent in Tirunelveli district of Tamil Nadu. Studies were carried out to evaluate the efficacy of botanicals, oil cake extracts, endophytic antagonists and chemicals in inhibiting the mycelial growth of  B. oryzae. Among the 15 botanicals, Lawsonia inermis (10 per cent) resulted in the reduction of maximum mycelial growth B. oryzae (65 per cent) over control followed by Pithecolobium dulce (60.56 per cent). Among the five oil cake extracts, coconut cake extract recorded the reduction of maximum mycelial growth of B. oryzae (71.89 per cent) over control at 10 per cent concentration followed by Neem cake extract (50.89 per cent). Among the 39 endophytic bacteria isolated 23 were Bacillus isolates and 16 were Pseudomonas isolates. Among the Bacillus and Pseudomonas strains BA2 (Ambasamudram) showed the reduction of maximum growth of B. oryzae (52.22 per cent) over control and PS1 (Sagayapuram) resulted in the reduction of maximum mycelial growth of B. oryzae (53.33 per cent) over control respectively under in vitro conditions. Among the chemicals, triazoles and strobilurin compounds showed highest reduction of mycelial growth of B. oryzae. The best performing botanicals, oilcake extracts, endophytic bacterial strains and chemicals were evaluated under pot culture and field experiments. The results from both pot and field experiment revealed that, hexaconazole followed by coconut oil cake extract and Lawsonia inermis recorded the least incidence of brown spot of rice respectively.