In the present study an attempt has been made to know the effect of surfactants on stain removal. On the basis of survey results, three popularly sellable surfactants in the local market and four common stains experienced by the working men and women were selected. White organic cotton fabric was stained with curry, grease, pickle and mud stains and aged for a period of 7 days to make them artificially old. Each stain was washed with selected three surfactants viz., Pure White with greenish granules (WG), Pure White (PW) and Pure White with bleu and orange granules (WBO) totaling to 3 washes with a gap of 24 hours. The change in the shade of the stain was assessed after every wash through spectrophotometer and it was found that off the three selected surfactants, PW was focused to be effective in removing the grease stain where as WBO in removing mud, pickle and grease stains compared to ‘WG’ surfactant.