The field experiment was conducted at Main Agricultural Research Station, UAS, Dharwad during kharif 2013to study the effect of chemical defoliator application on growth, yield parameters and quality of compact cotton genotypes.Treatments consisted of 12 combinations comprising of two genotypes (RAH-274 and SC-2028-22) as main plots, twospacing (45x10 cm and 45x15 cm) as sub plots with three chemical defoliator (Dropp Ultra @ 200 ml/ha, Ethrel @ 2000ppm and Water spray) as sub sub plot treatments. Genotype, RAH-274 under 45x15 cm spacing with Ethrel @ 2000 ppmproduced significantly higher seed cotton yield (2075 kg/ha) along with higher yield components than other treatmentcombinations. However, genotype SC-2028-22 recorded significantly higher leaf area (9.54 dm2/plant), LAI (1.75), numberof leaves per plant (3.1), leaf dry weight (14.1 g/plant) compared to RAH-274 at 18 days after defoliant spray (DADFS).Spacing of 45x15 cm recorded significantly higher growth parameters except LAI during all the stages of observationintervals over 45x10 cm. Among the defoliators, Ethrel @ 2000 ppm recorded significantly lower leaf area (3.01 dm2/plant),LAI (0.54), number of leaves per plant (1.4) and leaf dry weight (6.9 g/plant) compared to Dropp Ultra @ 200 ml/ha.Whereas, these parameters were significantly higher under control treatment. Interaction of SC-2028-22 at spacing of 45x15cm with water spray (control) recorded significantly higher leaf area, number of leaves and leaf dry weight except LAI overrest of the interactions.