Random Amplified Polymorphic DNA (RAPD) profiles for four cotton genotypes were generated with 19 random decamer primers. The primers generated 123 RAPD loci, of which 97 were polymorphic. The primers like GH-10, H-1, H12, L-13 and L-14 exhibited 100 per cent polymorphism. Maximum genetic distance was found between Jayadhar and LRA-5166 (53.29%), while the lowest genetic distance was between Abadhita and LRA-5166 (9.68%). The dendrogram of cotton genotypes showed two major clusters. The genotypes Jayadhar and Rahs-14 in one cluster and Abadhita and LRA-5166 in other cluster.