An experiment was conducted during rabi 2004-05 on medium black soils at Main Agricultural Research Station, University of Agricultural Sciences, Dharwad to study the influence of prolonged nitrogen application and sulphur nutrition on wheat and soil available nutrient dynamics at harvest. Grain yield and quality and nutrient uptake of wheat were not influenced significantly due to increasing number of splits from two (1/3 N as basal + 2/3 N at 1st node stage) to three (1/2 N as basal + 1/4  N at  tillering  +    1/4 N at milking). Crop responded to sulphur nutrition particularly as gypsum. Application of 50 kg ha-I S through gypsum recorded significantly higher number of productive tillers (77.66m-I), grain protein content (12.51%), sedimentation value (45.34 ml), grain yield (4.74 t ha-I) and N, P, K and S uptake (154, 28.70, 104 and 18.57 kg ha-1, respectively).