An attempt was made to study the general and specific combiningability in sesame (Sesamum indicum L.) through Line x Tester analysisby using ten lines and three testers. A total of thirty crosses weredeveloped and twelve quantitative traits were studied. The analysisof variance indicated the presence of significant variation among thegenotypes, parents and hybrids utilized for all the twelve traits exceptlength of capsules in the study. The analysis of variances for combiningability indicated that, mean squares due to females x males interactionwere highly significant for all the traits under study except length ofcapsule and 1000 seed weight. Based on the general combining abilityeffects, the line RT-346 and GT-10 was found to be a good generalcombiner for seed yield per plant and other parameters. Among thetesters, TKG-22 exhibited good combining ability for seed yield perplant, number of capsules per plant and days to maturity. The crosscombination GT-10 x DS-5 expressed the highly significant and positivesca effect for plant height, number of capsules per plant, days tomaturity, days to 50 % flowering, 1000 seed weight, oil content andseed yield per plant but negative sca effect for length of capsule andnumber of seeds per capsule. Also, the cross DS-21×TKG-22 wasfound to have significant and positive sca effects for more number oftraits like days to 50 % flowering, plant height, seed yield per plantand oil content. Hence these crosses could be utilized for yieldimprovement through heterosis and pedigree breeding.