An in vitro experiment was conducted on evaluation of chemicals, antibiotics, selected nutrients and bio-agentsagainst bacterial leaf spot of betel vine caused by Xanthomonas axonopodis pv. beticola in department of Plant Pathology,College of Agriculture , University of Agricultural Sciences, Dharwad. In vitro evaluation of bactericides, bio-agents andselected nutrients against the pathogen served as a guideline for the field level management of bacterial leaf spot diseasewhich was seriously affecting the betel vine production. Results revealed that, among those, K-cyclne at 750 ppmconcentration with the inhibition zone of 25.33 mm, Bordeaux mixture at 3000 ppm concentration (12.00 mm), K-cyclineat 500 ppm concentration with COC at 2000 ppm concentration (25.33 mm), CuSO4 at 0.5 per cent concentration(5.60 mm) and Pseudomonas fluorescens (11.00 mm) were found superior over rest of antibiotics, chemicals, combinationof antibiotics and chemicals, selected nutrients and bio-agents against Xanthomonas axonopodis pv. beticola, respectively.