Journal of FARM SCIENCES, Vol 31, No 1 (2018)

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Growth of Pseudomonas striata in liquid formulations as influenced by different concentration of additives, adjuvants and surfactants



An attempt was made to develop liquid formulations of Pseudomonas striata, a phosphate solubilizing bacteriausing different concentrations of additives, adjuvants, surfactants and to study its survival on seeds. The results indicatedthat growth of Pseudomonas striata was higher in liquid formulations compared to unamended basal medium. The promisingliquid formulation was used to treat maize and sorghum seeds at concentrations of 2 mL, 4 mL 6 mL and 8 mL kg-1 seed andthe treatment effect was compared with treatment of seeds with lignite based formulation @ 20 g kg-1. Liquid formulationtreated @ 2 ml kg-1 maize and 4 ml kg-1 sorghum seeds resulted in higher mean viable population of log10 6.72 CFU seed-1 andlog10 5.36 CFU seed-1 respectively. The least viable population was seen in treatment with lignite based formulationrecording population of log10 4.72 CFU ml-1 seed-1 on maize seeds and log 10 2.508 CFU ml-1 seed-1 on sorghum seedsrespectively. The cost involved in production of the best formulation was rupees 149 litre-1. This cost included the cost ofchemicals, cell protectants, packing material, depreciation cost of instruments, electricity cost and the labour cost. Lignitebased formulation coasted rupees 60 kg-1, and was inoculated @ 20 g kg-1 seed. The cost of this technology was about rupees1.2 kg-1 seed. As only 2 to 4 ml kg-1 seed of promising liquid formulation was required the total cost of using this technologywas less than one rupees per kg seeds.

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