A study was conducted in Central parts of Western Ghats of Virajpet taluk, Kodagu district to estimate carbonsequestration potential in soils of different land use systems. The land use systems viz., sacred grooves, coffee basedagroforestry-indigenous, coffee based agroforestry-exotic and paddy land use systems. The soil samples were collected atdifferent soil depths (0-15, 15-30, 30-60 and 60-90 cm). The results indicated variation of soil carbon fractions in differentland use systems under different depths. Among different land use systems, the higher mean value of Potassium DichromateOrganic Carbon (11.80 g kg-1), Potassium Permanganate Organic Carbon (1154.80 mg kg-1), Cold Water Extractable Carbon(525.00 mg kg-1), Soil Microbial Biomass Carbon (507.53 mg kg-1), Total Organic Carbon (43.00 g kg-1) and Total Carbon(43.51 g kg-1) content was observed in sacred groves whereas, highest mean value of Total Inorganic Carbon (0.61 g kg-1) wasobserved in paddy land use system and different soil carbon fractions decreases with increasing soil depths. The sequenceof distribution of different soil organic carbon fractions is more in sacred grooves followed by coffee based agroforestryindigenous,coffee based agroforestry-exotic and paddy land use system.