An experiment was conducted during the year 2016-17, in the naturally ventilated polyhouse to induce andobserve the mutation frequency, abnormalities and variation in growth and flowering quality of chrysanthemum genotypeby chemical mutagen viz., ethyl methyl sulphonate (EMS). Fifty per cent death of plant known to produce maximum rateof mutation in the population (LD50), among the treatments the survival per cent at treatment EMS 0.70 per cent for 1 h45 min treated plants were near to the LD50(55.83%). The increasing trend in chlorophyll mutants and other abnormalitieswas observed with increasing concentration of mutagen and its duration. Significantly maximum chlorophyll mutants,abnormal leaf and abnormal flowers were produced in treatment where the rooted cuttings treated with EMS 0.70 per centtreated for 1 h 45 min. Significantly reduced vegetative parameters and delayed flowering were recorded with increase indose and duration of chemical mutagen. Whereas, in control plant recorded significantly maximum survival, vegetativegrowth and early flowering. Among the genotypes, survival percentage was significantly maximum and delayed floweringwas reported in genotype Kattiraja (77.59 %). Whereas, minimum survival percentage and early flowering was recorded ingenotype Marigold (73.52 %). The other abnormalities and chlorophyll mutants didn’t vary significantly among thegenotypes. Among the interactions, genotype Marigold treated with EMS at 0.70 per cent for 1h 45 min. recorded maximumabnormalities and mutants with reduced growth parameters and delayed flowering attributes