Proteases are degradative enzymes which hydrolyse peptide bonds in proteins and generate amino acids andsmall molecular weight peptides. They are also called peptidases or proteinases. They are ubiquitous in nature. They arefound in all living organisms such as plants, animals and microbes. However, the microbial production of proteases ispreferred over other sources because microbes can be grown quickly and require a small place (Kauffman et al., 2007). Invitro studies were conducted to isolate protease enzyme producing bacterial isolates. Sixty eight proteolytic bacterialisolates were isolated from different sources using soy meal agar medium. Top ten isolates were selected based on enzymepotency. Finally the best isolate (PPB-3) was selected by calculating the protease enzyme activity. The highest extracellularprotease enzyme activity reached to a maximum by the isolate from green peas viz., PPB-3 (20.62 U/ml) after 48 h ofincubation. In the medium soy meal was used as the protein source. Since it contains 40 per cent protein, it induced theprotease production.