The efficacy of biocontrol agents on Macrophomina phaseolina (Tassi) Goid., which causes dry root rot of pigeonpea were studied. In dual culture technique both Trichoderma virens (PDBC TVS-2) and Pseudomonas fluorescens (PDBC Pf1) significantly inhibited the mycelial growth of M. phaseolina by 78.22 per cent and 76.66 per cent respectively. Among the three methods by which, the talc based formulations of these bioagents were applied, seed treatment (4g/kg seed) along with soil application (2 g talc powder mixed with FYM/pot) supported the maximum plant stand and less root rot incidence compared to other  treatment and found significantly superior to seed treatment (2g/kg seed). However, it was on par with soil drenching with carbendazim (0.1%). Seed treatment + soil application with T. virens recorded less root rot incidence (2.89%) among the four bioagents used.