A protocol for direct shoot regeneration without intervening callus phase was developed by using shoot tip culture on MS (Murashige and Skoog) medium for Sugarcane (Saccharum spp.) variety CoC-671. The study revealed that aseptically inoculated shoot tips of sugarcane in BAP (Benzylamino purine) @ 2.0 mg/l established the shoot induction and shoot multiplication was also observed at same concentration. At 2 mg/l of BAP (79.64%), the shoots were weak, tiny and non-separable, whereas at 1.0 mg/l of BAP (21.84%) rate of multiplication was low (5.46) but resulted in desirable quality, well grown, easily separable and healthy plantlets. The root induction was observed NAA (Napthalene acetic acid) @ 2 mg/l (70.00%) and complete plantlets were hardened and transferred to greenhouse for establishment with a survival rate of 72 per cent.