Among biotic stresses, stem and pod rot caused by Sclerotium rolfsii Sacc. is a major constraint for productionin most of the groundnut growing area. Persistence of the pathogen in soil and its wide host range often limit the effectivenessof chemical and cultural control of stem and pod rot signifying the role evaluation of genetic resistance. It is evident thatstem rot disease has significant effect on the yield. Hence, the simultaneous evaluation of the genotype for the disease aswell as yield potential would be more useful. One hundred and ten promising lines for Sclerotium rolfsii resistance in F3generation were evaluated in S. rolfsii sick plot by creating artificial inoculation condition using Augmented Design in rabi,2016 and summer, 2017. Stringent selection through artificial inoculation was carried out based on percent disease incidence.Most of the genotypes were susceptible to the selected isolate and the resistant reaction was observed only in fewgenotypes. Three lines viz., TMV-2 × AGL 168-3, Dh-86 × AGL 289-6 and TMV-2 × AGL 289-11 in F4 and two lines viz.,TMV-2 × AGL 168-3 and Dh-86 × AGL 635-7) in F5 generations exhibited disease incidence less than 10 %. Present studyindicated that response of F4 and F5 lines to stem rot disease was variable over the seasons. Resistance of these genotypesto S. rolfsii varied with the growth stages of the plant.